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KMID : 0545120090190050474
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 5 p.474 ~ p.481
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Production of 3-Hydroxypropionic Acid from Acrylic Acid by Newly Isolated Rhodococcus erythropolis LG12
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Lee Sang-Hyun
Park Si-Jae
Park Oh-Jin
Cho Jun-Hyeong
Rhee Joo-Won
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Abstract
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A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropolis LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an OD600 of 5. Further cultivation of R. erythropolis LG12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/I/h at 30C after 72h.
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KEYWORD
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3-Hydroxypropionic acid, acrylic acid, Rhodococcus erythropolis LG12, resting cell reaction
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